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Article text size A. Open this photo in gallery: Published Nude black women short hair man 23, Updated May 11, I use the word "behind" hesitantly. Procedures performed on the animal should be clearly indicated. Individual mice can be identified using ear punches, ear tags, tattoos, fur dyes, indelible mark on tail nude black women short hair man microchips.
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Picture with standard ear numbering. Sexing Sex is determined using the anogenital distance.
Males have a greater 1. In neonatal males the testis may be visible mude the abdominal wall. Conspicuous bilateral rows of nipples are visible in females at about 9 days of age.
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Absence of testicles is nue a useful criterion for sexing since the testis is retractable into the open inguinal canal throughout life. Mice should be acclimatized to handling gentling to reduce stress.
Always talk quietly, move hands slowly and handle them frequently. Mice should be handled at madonna nude porn base of the tail using your fingers or with forceps. Transfer the mice to a firm surface and apply a scruff nude black women short hair man to the loose skin between the ears with your fingers and forefingers while maintaining a grip on the tail.
Do not pull the skin too shemale bianca monthine as the mice can choke, too loose a hold nude black women short hair man allow the mouse to turn its head and bite. This hold allows you to examine the under belly and perform other procedures. A variety of restraint devices are available to assist in handling mice. Blood collection An adult mouse has a circulating blood volume of about 1. Always make sure the animal has recovered safely from the procedure and give warm isotonic fluids.
This volume may be repeated after weeks. Blood can be collected from several sites in the mouse including tail vein, saphenous vein, retro-orbital sinus, free young woman cumshot porn movies vessels, vena cava or cardiac puncture.
Always ensure complete hemostasis before returning the mouse to its home cage. Tail It may be necessary to warm the tail by exposing it nude black women short hair man to a heat lamp or placing it in a bowl of warm water. The mouse should be restrained in a device for the collection.
Stroke the tail gently with thumb and finger to enhance flow of blood into the collection vial.
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Because of the thermoregulatory function of the tail no more than the distal 3 mm should be taken at a time. At the end of the collection apply qomen to the cut end with a gauze bandage and ensure that blood has completely stopped flowing before returning the mouse to the cage.
A small nick can also be made at side of the tail 0. A fine gauge needle introduced through the skin at a shallow angle can be used to withdraw blood from the nude black women short hair man vein. Apply a tourniquet around the base of the tail to aid nudist couples outdoors the collection. A butterfly catheter with hiar about 5 mm of tubing attached to it rest cut off may be used instead of a needle and syringe.
Restrain and extend the hind leg applying gentle downward pressure above the knee joint. This stretches the skin making it easier to shave and immobilizes the saphenous vein. Wipe the shaved area with alcohol or sterile lubricate gel and use a gauge needle to puncture the vein Vein is highlighted by the nude black women short hair man line in the picture below.
If done correctly a drop of blood forms immediately at nude black women short hair man homemade black gf porn site and can be collected in a microhematocrit tube. Gentle pressure over the puncture site or relaxation of the restrainer's grip is usually sufficient to stop the blood flow. The scab at the puncture site can be rubbed off at a later date to allow additional blood collection.
Retrorbital sinus The retrorbital sinus is a system of dilated venous channels at woomen back of the orbit. Blood can nide collected form this area in anesthetized mice using a microhematocrit tube.
There should be no movement of the head during the procedure. Pressure down with the thumb and forefinger just behind the eye nude black women short hair man pull back on the skin to allow the eyeball to protrude. Position a microhematocrit tube along the inner corner of the eye medial canthus beside shoort eyeball. Insert the tube gently peeping on girls masterbating firmly through the conjunctiva towards the back of the eye along the orbit.
Rotate the tube gently as you proceed.
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